 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : In Vitro Propagation of Ginger ( Zingiber officinale Roscoe )) ผู้เขียน: Yama Raj Pandey,  นายชัยฤกษ์ สงวนทรัพยากร, ผู้ช่วยศาสตราจารย์ , นางอรดี สหวัชรินทร์, รองศาสตราจารย์, ดร.นิพนธ์ ทวีชัย, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractDisease free ginger plantlets were produced through tissue culture. The method for surface sterilization was soaking the emerging buds in distilled water containing 0.2% Tetracycline and 0.2% Metalaxyl for one and half hour before treated with 10% Clorox for 15 minutes followed by 5% Clorox for 10 minutes. Shoot tips were excised from the buds and planted onto MS medium supplemented with 3 mg/l BA. Eighteen percent of the explants were obtained as clean culture. The uncontaminated shoot tips were transferred to MS media supplemented with 5 mg/l BA for shoot growth . Shoot tips were then cultured on MS and 2 g/l Twin Forty ( a commercial fertilizer medium containing N, P2O5 and K2O 30:20:10 respectively) media with and without supplementing plant growth regulators. The supplemented plant growth regulators were 5 mg/l 6-benzylaminopuring ( BA ) in combination with 0.5 mg/l naphthaleneacetic acid ( NAA ) and 4 ml/l Surprise ( a concentrated liquid plant food which contains natural biopolymer with organic acid an inert substances). The purpose of the study was to sort out the sample and cost effective medium and plant growth regulator for in vitro propagation of ginger with high multiplication rate. All the cultures were incubated at 25 + 2o C and 12 hours photoperiod. Pseudostems cultured on MS basal medium supplemented with 5 mg/l BA in combination with 0.5 mg/l NAA produced the highest number of shoots with an average of 5.33 shoots per psuedostem after 5 weeks of culture. Similarly length of shoots was highest on the same medium . Twin Forty fertilizer medium without supplementing with any plant growth regulators produced the lowest number of shoots, leaves and length of shoots. The cost of materials per plantlet was lowest on MS and Twin forty fertilizer media supplemented with BA in combination with NAA. The results revealed that MS and Twin Forty media supplemented with plant growth regulators such as BA in combination with NAA are more effective than surprise and without supplementing any plant growth regulators for cost effectiveness and rapid multiplication. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effect of Planting Material on Growth and Seed Rhizome Yield of Ginger (Zingiber officinale Roscoe)) ผู้เขียน:Yama Raj Pandey, นายชัยฤกษ์ สงวนทรัพยากร, ผู้ช่วยศาสตราจารย์, นางอรดี สหวัชรินทร์, รองศาสตราจารย์, ดร.นิพนธ์ ทวีชัย, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf Abstract Meristem derived ginger plants cultivar khing Yai were micropropagated in vitro. Small rhizomes were produced from these plantlets under different photoperiods (8, 10, 12, 14 and natural daylength (12.01 – 11.19 hrs.)). The performance of small rhizomes produced from micropropagated plantlets compared with conventionally planting big rhizomes and direct transplanting of micropropagated plantlets was evaluated from growth, rhizome yield and rhizome branching. The conventionally propagated old rhizomes produced more fresh rhizome yield than rhizomes and plantlets from micropropagation but tillering and rhizome branching was more in rhizomes from micropropagation as compared with conventional method and direct transplanting. The result revealed that ginger plantlets produced through micropropagation should be planted for seed rhizome production under greenhouse conditions and after harvest these rhizomes should again be planted for two generations of multiplication which can then be used as mother plants for commercial ginger production. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Influence of Photoperiods on Dormancy and Rhizome Formation of Ginger (Zingiber officinale Roscoe)) ผู้เขียน:Yama Raj Pandey, นายชัยฤกษ์ สงวนทรัพยากร, ผู้ช่วยศาสตราจารย์, นางอรดี สหวัชรินทร์, รองศาสตราจารย์, ดร.นิพนธ์ ทวีชัย, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe effects of photoperiods on dormancy and rhizome formation of ginger (Zingiber officinale Roscoe) were studied, Ginger plantlets produced form tissue culture were transplanted for better establishment and acclimatization under greenhouse and open field conditions. Well established and acclimatized plants were exposed to 8,10,12,14 hours and natural (12.01-11.19 hours) photoperiods during October, 1995 to January, 1996. After adjustment of photoperiods in October, new tillers were continuously produced till January under 14 and 12 hours photoperiods. New tiers were not produced and plants started yellowing and dying in November under 8 hours photoperiod and in December under 10 hours and natural photoperiods. The crop had no dormancy under 14 and 12 hours but entered dormancy under 8,10 hours and natural photoperiods. The highest fresh rhizome yield was produced under 12 hours photoperiod but rhizome yield for seed purpose was highest under 10 hours and natural photoperiods. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Variability of French Bean in the Western Mid Hills of Nepal) ผู้เขียน:Yama Raj Pandey, Durga Mani Gautam, Resham Bahadur Thapa, Moha Dutta Sharma, Krishna Prasad Paudyal สื่อสิ่งพิมพ์:pdf AbstractVariability was studied among 18 exotic and indigenous French bean (Phaseolus vulgaris L.) genotypes collected from research centers, agro-vets and traditional farming villages of the western hills of Nepal. The collected genotypes were field evaluated at the Agriculture Research Station, Malepatan, Pokhara at 848 m above sea level during the summer season of 2010 with the objectives to assess the variability in the exotic and indigenous genotypes and their potential for utilization in improvement programs. The results of the study showed that the variability was higher in adaptation, vegetative growth, floral and pod characteristics. The plant survival at harvest was higher in pole-type than in bush-type beans ranging from 97.92 to 54.17% with means of 83.71% and 79.80%, respectively. Bush-type beans were earlier in flowering than pole-type beans ranging from 32 to 174.33 d with a mean of 35.76 and 76.61 d, respectively. Pod length and width were higher in pole- type beans than in bush-type beans ranging from 20.45 to 7.67 cm in length and 33.53 to 7.37 mm in width. The variability indicated that the collected genotypes were distinctly different. The results revealed that the French bean genotype in the mid hills of Nepal is highly diverse and could be considered as the secondary center of genetic diversity. The diverse genotypes should be conserved and utilized for varietal improvement. |